we use NBF for at least 16 hours, the next day after receiving they are put
in decal (mixture of formic acid and hydrochloric acid with formaldehyd) for
7-8 hours. Our biopsies are about 3-4 mm thick.
After acid decalcification enzyme-stains are not possible any more and the
iron-stain could be false negative. But we have good experiences with the
IHC and had even an improvement with some markers compared with EDTA-decal.
[mailto:firstname.lastname@example.org] Im Auftrag von Angela R.
Gesendet: Mittwoch, 24. September 2008 19:40
Betreff: [Histonet] Bone Marrow Biopsy Fixation
Have you fixed bone marrow biopsies in formaling and if so for how long
and what decal solution is best?
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