Hi Kathy-

We use a protocol which calls for a 10 second dip in 2% AAS.
Solution:
 5 mls of 3-aminopropyltriethoxysilane (Sigma A-3648)
250 mls of pure (99.5%) acetone

We first thoroughly clean and oven-dry slides. Dry slides are dipped in 2%
AAS for 10 seconds, rinsed in deionized H2O 3X, 5 dips each. Air-dry or oven
dry slides. Several racks of slides (up to 400 slides) can be run through
this solution. Slides should be OK at room temperature for at least 6
months.

I don't know if the time in silane is the problem.

Good Luck!

Lorraine Gibbs
Physiology & Biophysics
University of Washington
----- Original Message -----
From: "Kathleen J Savage" 
To: 
Sent: Thursday, September 06, 2001 7:10 AM
Subject: silane coated slides


> Hello all,  I am having a problem with tissue (skeletal muscle fibers)
> falling off silane coated slides as soon as any liquid in put on the
> slide. We coat the slides ourselves  following the procedure given by
> Sigma Chemical with the exception of dipping in silane for only 5 seconds
> instead of the suggested 2 minutes.  The 5 second dip in silane follows
> the original procedure of Maddox and Jenkins.
> I am wondering if just the time spent in the silane is the cause of my
> problems.  Any suggestions or comments are greatly appreciated.
> Kathy
>
>