I have used the Dako EnVision system on paraffin processed liver and kidney
biopsies without any problems.   The tissues were fixed in 10% buffered
formalin and antigen retrieval was not needed.  If you have any specific
questions please feel free to contact me.


-- Begin original message --
>
> Dear Histonetters,
>
> My lab faces a problem of IHC investigation of paraffin archives of
> nephrobiopsies.
> I'd be grateful for advices on contens of Ab panel and visualisation system
> for this purpose. Have somebody applied EnVision to paraffin sections of
> nephrobiopsies? If so, does EnVision offer advantages over other systems?
> Only DAKO's reagents are avaliable for us.
> I appreciate any help.
>
> Dr Y Krivolapov MD
> Associate Professor in Histopathology
> Head of Laboratory of Molecular Pathology and Cytogenetics
> Department of Haematology and Clinical Immunology
> Military Medical Academy
> St.-Petersburg Russia
-- End original message --

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first.
Mark Twain [Samuel Langhornne Clemens] (1835-1910)