Antigen Retrieval

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From:"Bennett, Catherine (Katie)" <> (by way of histonet)
Date:Wed, 20 Oct 1999 03:46:52 -0400
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Do all antigen retrieval procedures do the same thing?  In other words, can
an antigen retrieval procedure that is used in one immunohistochemical run
be used successively in another IHC run?  Or is it more a case of trial and
error to see which antigen retrieval will work in any given new IHC
procedure?  (I'm sure all is dependant upon what type of fixative was used
and what type of tissue is being stained.  Let's say for this query that
fixation and tissue type are the same, just primary antibody is different.)

Also, are there any big differences in terms of results using a commercial
citra antigen retrieval as compared to a recipe using a 1% zinc sulfate
solution (both are microwave protocols)?

As always, thanks in advance!

Catherine "Katie" Bresee Bennett
Sr. Technical Associate
Lovelace Respiratory Research Institute
Albuquerque, New Mexico

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