Tim

we also use the clone 3-12 for mice and monkeys, for rat FFPE we have
success with the polyclonal anti-human CD3 from DakoCytomation.
We usually do antigen retrieval with citrate buffer pH 6.0 for 20 min in
microwave. Dilution for the primary is 1:200 , incubation for 60 min.
Hope this helps.

Antje Marcantonio
Novartis Pharma AG
Basle, Switzerland