Snobird75@aol.com wrote:
> Does someone have a procedure for doing a gram stain, where 
> you don't use the 50% solution of xylene and picric acid 
> (Bouins).

Dear Snobird75 (whoever you are),

Where did you find a Gram staining method using either
xylene or Bouin's fluid (which does not contain xylene)?

The chemicals used in Gram staining are crystal violet,
iodine and potassium iodide. 

-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/