Re: Bone sampling

From:louise renton

Depends on the size of the sample needed and of course the specimen. What 
about using a Jamshidi(?sp) needle used for bone marrow sampling? The ones 
I've seen make a small core biopsy about 2mm diameter. but possibly there 
are larger ones out there. We used to get cores for  metabolic bone studies 
that were larger about 5mm, but I have no idea what they used to get them. 
How about finding a friendly orthopod who will let you look at his array of 

Best regards

Louise Renton
Bone Research Unit
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"

>From: Nick Bullough 
>To: "Histonet (E-mail)" 
>Subject: Bone sampling
>Date: Thu, 24 Oct 2002 15:13:04 +0100
>Can anyone help with a nice little problem I have?
>I need to sample both cortical and cancellous bone for histology and RNA
>extraction. I've seen a dowel cutter that can be attached to a drill to 
>cores of bone that will be ideal for what is needed.
>My problem is that the health & safety people are very nervous about
>aerosols and other contamination (bone dust). My idea was to enclose the
>cutting in a box or behind a shield as it will be almost impossible to do
>this within a safety cabinet (and there is still the problem of spray from
>the cutter.
>How do other people deal with bone sampling? any good ideas very gratefully
>Best regards
>Nick Bullough BSc(Hons) AIBMS
>Pharmagene plc
>2 Orchard Road
>Herts SG8 5HD
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