It would be xylene/acetone mixture, right? And not Bouin's/Formalin? In the
original query, you mentioned getting rid of Bouin's/xylene. So what is it
to be?

Salvacion Delovino

> ----------
> From: 	Snobird75@aol.com[SMTP:Snobird75@aol.com]
> Sent: 	Friday, October 25, 2002 12:53 PM
> To: 	jkiernan@uwo.ca; histonet@pathology.swmed.edu
> Subject: 	Re: Gram Stain
> 
> Dear John
> After you use crystal violet, Gram Iodine  and counterstain, towards the
> end 
> of the stain you have to use a 50% solution of xylene and acetone. Then
> take 
> it into the xylene and coverslip. There is a step where Bouins and
> Formalin 
> mixture is used.
> I am trying to get rid of the xylene/acetone step. I don't have the
> procedure 
> at home with me. Its just a stain that the lab uses where I work.
> Thank you 
> sandi miller HT
> USAMRICD Research
> Maryland
>