Detecting GFP in Autofluorescent Bone Samples

From:Romy Thomas

Dear All,
Here is the problem:
Strong autofluorescence of bone masking any underlying GFP signals from
(We have tried the dual chroma filter of FITC & Texas red without much
Here are the questions:
1) What is the best way to fix and section bone samples to detect GFP
2) Any suggestions on the appropriate filter and source would be great
3) Any opinions on reporter gene signals that survive EDTA decal procedures?

Many thanks
Romy Thomas

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