Dear All,
Here is the problem:
Strong autofluorescence of bone masking any underlying GFP signals from
cells.
(We have tried the dual chroma filter of FITC & Texas red without much
improvement)
Here are the questions:
1) What is the best way to fix and section bone samples to detect GFP
signals?
2) Any suggestions on the appropriate filter and source would be great
3) Any opinions on reporter gene signals that survive EDTA decal procedures?

Many thanks
Romy Thomas