Re: tissue left out
Good morning,
There are a few recipes for this problem. Sanderson's fluid, Ruffer's
solution and an alcoholic/glycerol mixture. All usually say the leave
overnight and then process from dehydration step.
Sanderson's :
96% ethanol 30 parts
1% formaldehyde 50 parts
5% aq. sod. carbonate 20 parts
Ruffer's :
Water 50 parts
Abs alcohol 30 parts
5% sod. carbonate 20 parts
Taken from Bancroft and Stevens, Chapter 3(4th edition) (also see Culling,
Allison and Barr):
70% ethanol 70 cm3
glycerol 30cm3
diothionate 1g
Use a section adhesive after cutting these samples
Evelyn Kaplan
Dept of Pathology,
Sultan Qaboos University,
Muscat, Oman
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