RE: High Iron Diamine stain for sulphomucins

From:"Johnston, Kathy"

This is what I have found, although I believe the chemicals to be quite
toxic to use.


Kathy Johnston
Tech II - Special Stains
Anatomic Pathology - Foothills Medical Center
Calgary Laboratory Services
Ph - 403-944-4760
Fax - 403-270-4093

-----Original Message-----
From: Martin, Ronald []
Sent: Friday, October 04, 2002 12:14 PM
Subject: High Iron Diamine stain for sulphomucins

Fellow techs,
Does anyone have a procedure they are willing to share for a High Iron
Diamine stain for sulphomucins? This is new  to me so I will new information
regarding  times, concentrations, chemicals etc. We are trying to
differentiate between sulfated and carboxylated components in mouse
Thanks in advance,
Ron Martin
Research Associate

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