This is what I have found, although I believe the chemicals to be quite
toxic to use.

http://www.bris.ac.uk/pathandmicro/cpl/hidab.html

Enjoy!

Kathy Johnston
Tech II - Special Stains
Anatomic Pathology - Foothills Medical Center
Calgary Laboratory Services
Ph - 403-944-4760
Fax - 403-270-4093
kathy.johnston@cls.ab.ca


-----Original Message-----
From: Martin, Ronald [mailto:Ronald.Martin@umassmed.edu]
Sent: Friday, October 04, 2002 12:14 PM
To: histonet@pathology.swmed.edu
Subject: High Iron Diamine stain for sulphomucins


Fellow techs,
Does anyone have a procedure they are willing to share for a High Iron
Diamine stain for sulphomucins? This is new  to me so I will new information
regarding  times, concentrations, chemicals etc. We are trying to
differentiate between sulfated and carboxylated components in mouse
stomach/intestine.
Thanks in advance,
Ron Martin
Research Associate