This is my table:

You have your 2 stock buffer solutions:
1.   M/15 sodium phosphate dibasic (Na2HPO4) - dissolve 9.465 g in 1L dH2O.
2.  M/15 potassium acid phosphate (KH2PO4) - dissolve 9.08 g in 1L dH2O.

To make the amounts of each solution follow the below table:

pH		M/15 KH2PO4		M/15 Na2HPO4
			mL				mL
8.0			4				96
7.6			12				88
7.2			27				73
6.8			50				50
6.4			71				29
6.0			88				12
5.6			95				5

Hope this helps!

Kathy Johnston
Tech II - Special Stains
Anatomic Pathology - Foothills Medical Center
Calgary Laboratory Services
Ph - 403-944-4760
Fax - 403-270-4093
kathy.johnston@cls.ab.ca

-----Original Message-----
From: marina goumenou [mailto:mtg67@hotmail.com]
Sent: Saturday, October 05, 2002 2:26 PM
To: histonet@pathology.swmed.edu
Subject: Buffer recipe



Hello to everybody!

Your suggestions for my May Grunwald - Giemsa problems were really very 
helpfull. However, I stained my slides with dye solutions in Soerensen 
buffer pH 6,8 but they appeared to be too blue. So I intent to use even 
lower pH value (something like 6,0 or 6,2). Does anybody have a recipe for 
apropriate buffer (Soerensen or anything you think) in these pH values?
Thanking you in advance
Best Regards
Marina Goumenou
Athens - Greece


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