RE: Buffer recipe
This is my table:
You have your 2 stock buffer solutions:
1. M/15 sodium phosphate dibasic (Na2HPO4) - dissolve 9.465 g in 1L dH2O.
2. M/15 potassium acid phosphate (KH2PO4) - dissolve 9.08 g in 1L dH2O.
To make the amounts of each solution follow the below table:
pH M/15 KH2PO4 M/15 Na2HPO4
8.0 4 96
7.6 12 88
7.2 27 73
6.8 50 50
6.4 71 29
6.0 88 12
5.6 95 5
Hope this helps!
Tech II - Special Stains
Anatomic Pathology - Foothills Medical Center
Calgary Laboratory Services
Ph - 403-944-4760
Fax - 403-270-4093
From: marina goumenou [mailto:email@example.com]
Sent: Saturday, October 05, 2002 2:26 PM
Subject: Buffer recipe
Hello to everybody!
Your suggestions for my May Grunwald - Giemsa problems were really very
helpfull. However, I stained my slides with dye solutions in Soerensen
buffer pH 6,8 but they appeared to be too blue. So I intent to use even
lower pH value (something like 6,0 or 6,2). Does anybody have a recipe for
apropriate buffer (Soerensen or anything you think) in these pH values?
Thanking you in advance
Athens - Greece
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