RE: biopsy runs
From: | David Taylor Manager |
Dear Jennifer, If your biopsies are cutting as you describe, then either
they are over-heated, over-processed or under-fixed. We use a number of
"short cycles", depending on circumstances and tissue types. You need to
determine what you have and therefore what you need, to process tissue
well. If you have a well fixed (24 hours) 1-2mm biopsy specimen then a
2-3 hour cycle will do nicely. The cycle length will also depend upon
the processing technology you have at hand. If you use a Leica TP1050,
VIP1000, 2000, 3000, or a Shandon PathCentre, etc, then the pressure,
vacuum , heat and circulation options will reduce times. It will also
depend upon how often you change your reagents in the processor. Listed
below are the "short cycles" we use on our Leica TP1050's.
N.B. We have 2 TP1050's called Ken & Barbie, its odd but Ken does our
breast/ fatty runs and Barbie does all the rest of the work.
For the Leica TP1050 tissue processors, Ken and Barbie, (both have same
menu).
Program 1. 2 Hour rapid processing cycle, for very small biopsies, well
fixed.
(Run time = 2 hours )
Reagent Minutes Temp °C P/V Drain
Stirrer
10% aqueous formalin 0 50 P/V
80 sec. on
10% aqueous formalin 0 50 P/V
80 sec. on
Absolute alcohol 5 50 P/V
80 sec. on
Absolute alcohol 5 50 P/V
80 sec. on
Absolute alcohol 5 50 P/V
80 sec. on
Absolute alcohol 5 50 P/V
80 sec. on
Absolute alcohol 18 50 P/V
80 sec. on
Xylene 5 50
P/V 80 sec. on
Xylene 5 50
P/V 80 sec. on
Xylene 19 50
P/V 80 sec. on
Paraffin wax 5 62
P/V 120 sec. on
Paraffin wax 5 62
P/V 120 sec. on
Paraffin wax 12 62
P/V 120 sec. on
Program 2. 3 Hour rapid processing cycle, small biopsies, just about
fixed.
(Run time = 3 hours 00 minutes)
Reagent Minutes Temp °C P/V
Drain Stirrer
10% aqueous formalin 5 50 P/V
80 sec. on
10% aqueous formalin 19 50 P/V
80 sec. on
Absolute alcohol 5 50
P/V 80 sec. on
Absolute alcohol 5 50
P/V 80 sec. on
Absolute alcohol 5 50
P/V 80 sec. on
Absolute alcohol 10 50
P/V 80 sec. on
Absolute alcohol 20 50
P/V 80 sec. on
Xylene 5 50
P/V 80 sec. on
Xylene 10 50
P/V 80 sec. on
Xylene 25 50
P/V 80 sec. on
Paraffin wax 5 62
P/V 120 sec. on
Paraffin wax 10 62
P/V 120 sec. on
Paraffin wax 25 62
P/V 120 sec. on
For the Leica TP1050 tissue processors, Ken and Barbie.
Program 3. 3 ½ Hour processing cycle, routine colonic bx's etc..
(Run time = 3 hours 30 minutes)
Reagent Minutes Temp °C P/V
Drain Stirrer
10% aqueous formalin 5 50
P/V 80 sec. on
10% aqueous formalin 17 50
P/V 80 sec. on
Absolute alcohol 5 50
P/V 80 sec. on
Absolute alcohol 5 50
P/V 80 sec. on
Absolute alcohol 10 50
P/V 80 sec. on
Absolute alcohol 15 50
P/V 80 sec. on
Absolute alcohol 25 50
P/V 80 sec. on
Xylene 5 50
P/V 80 sec. on
Xylene 20 50
P/V 80 sec. on
Xylene 25 50
P/V 80 sec. on
Paraffin wax 5 62
P/V 120 sec. on
Paraffin wax 15 62
P/V 120 sec. on
Paraffin wax 25 62
P/V 120 sec. on
Hope this is of use, David.
David Taylor
Laboratory Manager
Drs King & Mower
Adelaide, Australia
-----Original Message-----
From: Jennifer Englin
Sent: Saturday, 29 September 2001 06:01
To: histonet@pathology.swmed.edu
Subject: biopsy runs
We are interested in trying out some short biopsy runs on our tissue
processor. We have tried a couple of different runs and our GI biopsy
are dry and chatter when we cut them.
If you have a good processing schedule that seem to work well can you
please fax it to me.
Thanks in advance!!!!!
PS: I Had a great time in Charlotte- Thanks everyone for all the hard
work you did and especially thanks to those who had the courage to come.
Sincerely,
Jennifer Englin HT(ASCP), PA
Rice Memorial Hospital
Willmar, MN
Fax #(320)231-4503
Phone#(320)231-4574
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