Help!!  I am having a problem with background staining with Kappa and
Lambda.  I know what the problem is but do not know how to solve it.  I am
told that it is a IgG problem but how do you block out the background??  Our
current method is as follows:  We use Cell Marque (monoclone) antibody.  No
heat retrieval.  We use Protease II for 8min. and incubate for 32min.  It
looks great except for the background staining.  Does anyone have any
suggestions???????

Sherry Smith
Presbyterian Hospital
Charlotte, NC


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