Michelle:

Does he want you to destain the H&E first?  When we have limited samples we
frequently do a GMS/H&E.  This involves ddoing a traditional GMS and then
instead of counterstaining with light green we use our H&E procedure with
step 1 being the hematoxylin and following our protocol down to xylene.

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Rd., N.E.  MS-G32
Atlanta, GA  30333 


-----Original Message-----
From: Michelle D. Moore [mailto:tmhpath@amigo.net]
Sent: Wednesday, October 10, 2001 10:03 AM
To: Histonet Server
Subject: GMS stain on an H&E


Hello fellow histonetters. Hope everyone is having a good morning. Quick
question for you, my pathologist has requested me to do a GMS stain from one
of my H&E sections this is a first for me and I don't know how to go about
this. The sample they collected for us was so small to begin with that even
when I did a GMS off the block it's not demonstrating what the doc is
looking for. He says he can see some areas on the H&E that he can't
distinguish between fungus and some other bacteria. HELP I have never tried
this before and would like your input. Thank you in advance for you time and
help. I'm glad to have a place that I can go for help like this since I'm in
such a rural area. Have a wonderful day.