Re: Declere and Tissue Adhesion

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From:"J. A. Kiernan" <> (by way of histonet)
To:histonet <>
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On Mon, 23 Nov 1998, Cell Marque Corporation wrote:

> The Declere protocol calls for the following:
> A) 3 micron thickness of sections on positively charged slides.
> B) Dry 1 hour in an incubator which has been properly calibrated at 58 C.
> C) Use either autoclave (metal pressure cooker with conventional heat
> source), conventional steamer, or other conventional heat source.
> I will be happy to address any additional questions.

   1. What does Declere contain?
   2. How does it work?
   3. Are there published comparisons (in
      refereed journals) with other procedures?

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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