We have used the Dako CSA kit successfully. We were able to detect
GFAP/astrocytes in long term (~ 5 years) NBF-fixed tissue without HIER.
This was not the case with LSAB or ABC dection techniques as used in our
hands. We have not tried the Dupont TSA kit.

	Regards from Liverpool,

	Daniel

************************
Daniel Brotchie
Unit of Ophthalmology
Department of Medicine
University of Liverpool
U.K.

On Mon, 23 Nov 1998 ppyle@notes.mdacc.tmc.edu wrote:

>Hi Histonetters,
>I wanted to ask if any one had had an success with either the Dako or Du
>Pont Life Sciences tyramise signal amplification (TSA) kits?  We want to
>increase signal with a research antibody.  Anyone had any success??
>Thanks for your input!
>
>Pam Pyle
>Neuropathology
>University of Texas M D Anderson Cancer Center
>713-792-7936
>ppyle@notes.mdacc.tmc.edu
>
>
>
>