casein
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
Purchase from Sigma, purified casein powder. There may be other sources
Dulbeccos PBS is available from Sigma in large volume, powder form also.
Cat #D5652.
Tacha method 5 gm in Dulbeccos PBS (any PBS probably will work)
0.5 g thimerosal
in 1 liter DPBS
Stir overnight, adjust pH to 7.4, store at 4C.
Reference: Tacha DE, et al Casein reduces nonspecific background
staining in immunolabeling techiques J Histotechnology
15:137-132, June 1992
Gayle Callis
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