Re: casein information needed
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From: | Mary Vaughan <mvaughan@sc3102.med.buffalo.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi Kathy,
There was an article in the J Histotechnology [J
Histotechnology/Vol. 15, No. 2/ June 1992] about Casein. They recommended
0.5% I tried it and in a series of experiments, reduced the concentration to
.03% [in PBS]. YES... this does make a difference. It's a terrific block [I
use 30 min.], AND it's non species specific, so you can use it with almost
every antibody [remember I said almost, depends on what the antibody is
against]. I use it routinely and at this dilution. When I used the 0.5%, I
lost some specific reaction. I also use it in my washes if I must use frozen
sections. Hope you find this helpful. I use Sigma #C-5890.
At 09:01 AM 11/4/98 -0500, you wrote:
>Good morning all!
>I am posting these questions for a research tech here in the department.
>What is a good source for ordering casein? What diluent do you use
>and at what dilution is casein most often used for IHC?
>Thank you
>Kathy
>************ Thank you ************
>* Kathy Oprea *
>* University of Georgia *
>* College of Veterinary Medicine *
>* Athens, GA 30602-7388 *
>* KOPREA@calc.vet.uga.edu *
>The worker who's never said "Thank
>God it's Friday," probably never
>really worked.
>************************************
>
>
Best Regards,
Mary Vaughan HT(ASCP)
Roswell Park Cancer Institute
Pharmacology + Therapeutics
Elm + Carlton Sts. CDC-121
Buffalo, NY 14263
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