rodent tissue peroxidase quenching

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From:Gayle Callis <> (by way of histonet)
To:histonet <>
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Some cell surface markers are very sensitive to methanol/hydrogen
peroxide quenching, reducing the staining of antigenic site, particularly
on frozen sections.  This is per PharminGen, they recommend using
hydrogen peroxide in PBS, and after talking to them, found out
they use Dako peroxidase block containing 0.03% hydrogen
peroxide in a buffer, with a bit of azide.

We now use this blocker with great success on frozens and paraffin sections
of murine/rat tissue.  It doesn't create bubbles that is a problem for
eating frozens alive, or with capillary gap and/or Shandon coverplate

Hope this helps
Gayle Callis

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