Re: [Histonet] Antibodies for frozen mouse sections

From:"Andrea T. Hooper"

I use the neutrophil marker Gr-1 from BD Pharmingen with fabulous 
success on frozen sections. It is actually a granulocyte marker not 
just neutrophils. Clone RB6-8C5. Use spleen as a positive control. In 
my hands it works great anywhere from 1-10 ug/ml depending on my 
detection system. I have not tried it on paraffin.

- Andrea

>At 10:00 AM 11/2/2005, you wrote:
>>Hi, I am staining some frozen mouse NALT and cervical lymph node
>>sections with a number of antibodies, CD4, CD8, B220, CD11c (all from
>>Pharmingen) and F4/80 (Serotec) and the staining is working fine (thanks
>>to Gayle Callis!!). However I want to start staining for some more cell
>>markers and I was wondering if anybody could give me details of primary
>>antibodies (dilutions etc) and where you get them from, that are good
>>for staining natural killer (NK) cells, ICAM-1 (CD54), CD5, MadCAM-1,
>>VCAM-1, PNAd and a neutrophil marker, i.e. Ly-6G.
>>Thanks very much in advance.
>>Lindsay Hall
>>Wellcome Trust Sanger Institute
>>Wellcome Trust Genome Campus
>>CB10 1SA

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