Re: [Histonet] antibody
What do you mean the secondary antibody's isotype is IgG (H+L)? You
mean they are anti-IgG (H+L)??
"Anti-IgG (H+L)" means anti-IgG (all isotypes) but reacts with both
heavy and light chains. So if I understand you correctly, then yes
these antibodies will react with your IgG1 and IgG3.
If at all unsure I strongly recommend you talk to the technical
center of the company who made the antibody or get the specification
sheet for your secondary.
>I have ordered two primary antibodies, their isotypes are IgG1 and
>IgG3 repectively. In our lab, there are some secondary antibodies,
>their isotypes are IgG (H+L), so I wonder if I use these secondary
>antibodies for immunofluorescence.
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