Re: Mouse lens/cataracts - membranes!!

From:Barry Rittman

The lens as it is dense will often stain different colors with stains such
as Mallory or van Gieson depending on the amount of time that you stain and
on different densities. Can see the same effects in teeth with these stains
depending on length of time staining.
I do not believe that the different colors in the lens are of real value in
diagnosis as I think that you will find that they are generally not
completely reproducible.

At 03:10 PM 11/22/2001 +1100, you wrote:
>Dear All, my name is Monica & I'm working on a mouse knockout
model(genetically modified) that develops cataracts at about 35 days of
age. I'm particularly interested in looking at any alterations in protein
or water distribution in the cataractous lens, also at the membranes of the
lens cells/fiber cells and if possible the extracellular space. I have
tried using Mallory's triple stain which has showed up some remarkable
differences between pre and post cataract lenses, but I don't have an
explanation for what each colour means as it's conventionally used for
collagen staining. Any ideas on the best stain to use to look at these
features or an explanation of the Mallory stain in this application would
be appreciated....Cheers,
>Monica (Melbourne Uni. OZ)
>    **********************************************************************
           BRUCE ABALOZ                           PH:61383446282
>HISTOLOGIST                            FAX:61383447909 
>DEPT.of ZOOLOGY                        EMAIL:
     This electronic message and all contents contain information which may be
>privileged, confidential or otherwise protected from disclosure.The
information is intended to be for the addressee only. If you are not the
addressee, any disclosure, copy, distribution or use of the contents of
this message is prohibited. If you have received this electronic message in
error, please notify us immediately and destroy the original message and
all copies.

<< Previous Message | Next Message >>