RE: direct peroxidase for CD31
Dear all the Histnet,
I'm very gratful and your time that you donate to response to my questions
about direct peroxidase for CD31.
I was able to get information from a former Tech. before me that have done
CD31 (but no longer in this lab). It turn out that this tech called a
"direct peroxidase" is because he was pretreated the slides before adding
CD31. The rest are the same steps (i.e. Biotinlyate, ABC step, DAB).
Futhermore, a purified anti-CD31 was purchased from the Pharm. In the end,
the whole procedure for CD31 that the former Tech did was NOT a direct
Most of the time I only do histolgy for the people in this lab is to from my
own knowledge and most of the help from other former lab that I worked
before and from everyone in Histonet info. The only problem is whom I
process the tissue for (post-doc, etc.). Especially for a person with narrow
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