Re: direct peroxidase for CD31

From:Thu-Thao Pham

No, he doesn't.


>From: rueggp 
>To: Thu-Thao Pham 
>CC: DAKOTechServ@dakousa.com, histonet@pathology.swmed.edu
>Subject: Re: direct peroxidase for CD31
>Date: Tue, 20 Nov 2001 18:08:11 -0700
>
>in order to direct peroxidase you will have to find or conjugate the cd31
>antibody yourself with peroxidase.  does your post-doc know this?
>patsy ruegg
>
>Thu-Thao Pham wrote:
>
> > I'm greatful and thankful to everyone that have taken their time to help 
>me
> > out with the CD31 method.
> >
> > I have a problem with a post-doc in the lab that wanted me to do CD31 on 
>a
> > mouse skin tissue. However, a tech. before be got fired and the hios lab
> > notebook cannot be found. In any case, a post-doc wanted to do a direct
> > peroxidase staining, because he said it faster and produce less 
>background
> > than using a ABC or LSAB2 method. He don't understand about IHC and he
> > cannot have an open minded about modification. I have give this post-doc
> > every single information from all of you that response to my questions 
>about
> > CD31, but he refused to go through with any method, but "direct 
>peroxidase."
> > He said that direct peroxidase method is VERY basic and that everyone 
>should
> > know and know how to do it (including myself), but why doesn't he, 
>himself
> > tell me how it's suppose to work?
> >
> > My questions, is does anyone know anything about direct peroxidase and 
>how I
> > can approached to do CD31? A post-doc asked me to email around and asked 
>all
> > of you to show me how to do direct peroxidase for CD31.
> >
> > I need to talk to my PI about this Post-doc and the rule in this lab, if 
>I
> > responsibility is to do histology for everyone in this lab.
> >
> > Thao
> > >From: DAKOTechServ 
> > >To: histonet@pathology.swmed.edu
> > >Subject: RE: DAKO kit for CD31
> > >Date: Tue, 20 Nov 2001 13:28:53 -0800
> > >
> > >Dear Histonet,
> > >
> > >First, I would like to say "Thank you" to Bob Meyer and Patsy Ruegg for
> > >your
> > >replies to this question.  Bob, you are correct, the LSAB and LSAB2 
>systems
> > >are designed for mouse and rabbit primaries.  The LSAB+ is designed for
> > >mouse, rabbit, and goat primaries.  The "2" in LSAB2 denotes our second
> > >generation of the LSAB system.  There were some improvements to the 
>LSAB
> > >system that required a second generation status.  Please feel free to
> > >contact me directly if you would like more information.
> > >
> > >Sincerely,
> > >Danielle Mach
> > >Technical Service Specialist
> > >DAKO Corporation
> > >800-424-0021
> > >
> > >-----Original Message-----
> > >From: r-meyer2@northwestern.edu [mailto:r-meyer2@northwestern.edu]
> > >Sent: Tuesday, November 20, 2001 10:56 AM
> > >To: histonet@pathology.swmed.edu
> > >Subject: Re: DAKO kit for CD31
> > >
> > >
> > >The DAKO LSAB system is for use with primary antibodies produce in 
>mouse or
> > >rabbit and is a labelled streptaviding-biotin system.  The LSAB2 system 
>is
> > >also
> > >a labelled streptavidin-biotin system for use with primary antibodies
> > >produced
> > >in mouse or rabbit, but it is optimized for use with DAKO N-Series 
>ready to
> > >use
> > >primary antibodies. The LSAB+ system is for use with primary antibodies
> > >produced in mouse, rabbit, or goat.  And I am not a salesman for DAKO 
>just
> > >use
> > >a lot of their products.
> > >
> > >Bob Meyer, HTL
> > >Northwestern University
> > > >
> > > > I looked through the catolog and don't understand the difference 
>between
> > >2
> > >
> > > > catolog:
> > > >
> > > > cat. K0681
> > > > Universal DAKO LSAB (labelled Streptavind-Biotin) system, Peroxidase 
>for
> > >use
> > > > with rabbit or mouse antibody.
> > > >
> > > > cat. K0675
> > > > Universal DAKO LSAB2 (labelled Streptavind-Biotin) system, 
>Peroxidase
> > >for
> > > > use with rabbit or mouse antibody.
> > > >
> > > > Thao
> > > >
> > > > _________________________________________________________________
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> > > >
> > > >
> > > >
> > >
> > >
> >
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>


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