Re: direct peroxidase for CD31
Okay, I asked the post-doc that question and he said he don't know. His only
response was that the former tech said direct peroxidase and he incubate the
CD31 overnight at 4oC and it worked for his tissue.
I'm just getting tire of this. I'm just go ahead and do what all of you
recommanded (i.e using a DAKA LSAB2 + kit).
Many thanks, again for everyone out there for your help.
Could the post-doc be meaning for you to use a secondary antibody conjugated
to HRP (peroxidase) as opposed to using a biotin amplification indirect
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