>Date: Mon, 24 May 1999 12:15 -0400 (EDT)
>From: nelso019@mc.duke.edu
>Subject: Eosinophil granule stain
>To: histonet@pathology.swmed.edu
>Mime-Version: 1.0
>
>     Does anyone know of a stain for eosinophils? I tried Luna's method and
>     the P.I. was not pleased. I understand there was one in the Journal of
>     Histotechnology a couple of years ago. Any suggesstions would be
>     helpful.
>
>     Thanks in advance,
>     Ramona Nelson
>     DUMC Dept. of Pathology
>     nelso019@mc.duke.edu
>     919-684-6209
>

Ramona,
	Try this stain, reliable and gives good images.
Ian.

CARBOL CHROMOTROPE.  (Eosinophils.)
	Lendrum. 1944.  J.Path.Bact. 56.  441.
	Simple and selective technique for eosinophils. The presence of
phenol lowers the pH of the stain increasing the selectivity for the
eosinophilic granules.

STAIN.
  Phenol.  =  1 g.  (Melt in a flask under hot water.)
  Chromotrope 2R.  =  0.5 g.  (Add to the melted phenol and mix.)
  Distilled water.  =  100 ml.  (Dissolve the dye sludge.)
  The stain keeps for up to 3 months.

METHOD.
1.) Bring sections to water.
2.) Counterstain nuclei with Haemalum. (If required - looks good.)
3.) Blue in running tap water.
4.) Carbol Chromotrope.  =  30 mins.
5.) Wash in running tap water.
6.) D.C.M.

RESULT.
	Eosinophil granules  =  Bright red.
	Nuclei.                    =  Blue/black.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk