EM on Nerve biopsies

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From:Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
To:HistoNet@Pathology.swmed.edu
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Date:Tue, 25 May 1999 09:03:25 +0000
Content-Type:text/plain; charset="us-ascii"

>Date: Mon, 24 May 1999 19:13:16 EDT
>From: RhodaRocks@aol.com
>Subject: EM on Nerve biopsies
>To: histonet@pathology.swmed.edu
>Mime-Version: 1.0
>
>
>
>Can anyone tell me how long the nerve should stay in the 2% gluteraldehyde
>for fixation?  When should it be transfererred to buffer for EM?
>
>Annette HT ASCP
>Kaleida Health Buffalo, NY
>

Annette,
	Presuming the nerve is an EM size block, 1-2 hours should be enough
for adaquate fixation. Into buffer wash then post fix with osmium.
Ian.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk





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