Re: [Histonet] Cryostat sections with small bubbles
I have never heard of hot plating cryostat sections. You may be boiling the
moisture under the section forming bubbles or do you air dry them well
first? Also -35 C seems too cold for brain sections, you may be getting some
I'm sure there'll be others with helpful information for you.
Hamilton, Ontario, Canada
----- Original Message -----
From: "Emily Jane Wiesner-Camm"
Sent: Tuesday, June 14, 2005 10:36 AM
Subject: [Histonet] Cryostat sections with small bubbles
> Hi All,
> I was wondering whether anyone can let me know why small bubbles appear
> underneath my rat brain sections when I cut them on a cryostat (at -35)
> when they appear perfectly flat before placing them on a hotplate. How
> they can be avoided?
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