Confused????

Isn't haematin like litmus and changes colour in acid and alkali
solutions? Doesn't the mordant act as a 'key' to the proteins and
haematin causing the dye to 'bind'? So why, if the iron haematoxylin is
in an acid 'environment' does it not require 'blueing'?

-----Original Message-----
From: Bryan Llewellyn [mailto:llewllew@shaw.ca] 
Sent: 15 June 2005 02:29
To: Histonet
Subject: Re: [Histonet] iron hematoxylins[Scanned]

I have blued Weigert's iron hematoxylin in the past, although I didn't
do it 
as a routine.  I wondered if the acid in the Weigert's solution affected
the 
colour and I tried it to find out.  Blueing does deepen the colour,
although 
not as significantly as with alum hematoxylin.  However, the acid
solutions 
I used afterwards in Masson's trichrome undid it and the end result was
the 
same.  It is useful if the iron hematoxylin is used alone without a 
counterstain, perhaps on smears for amoebae, but otherwise not.  Other
iron 
hematoxylin stains of the Heidenhain type do not need blueing.  The
mordant 
does that.

Bryan Llewellyn



----- Original Message ----- 
From: "Lee & Peggy Wenk" 
To: "'Steven Coakley'" ; 

Sent: Tuesday, June 14, 2005 5:07 PM
Subject: RE: [Histonet] iron hematoxylins


> I've never blued after a Weigert iron hematoxylin stain. Nuclei are
either
> black, or they aren't. If they are not, then the iron hematoxylin is
old
> (more than a couple days old), and putting the tissue into an alkali
> solution won't bring the color back.
>
> I'm curious, why are you bluing? What is the purpose? Maybe then I can

> help.
>
> Peggy Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven
> Coakley
> Sent: Tuesday, June 14, 2005 10:49 AM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] iron hematoxylins
>
> Does anyone know if using a bluing agent after weigerts iron
hematoxylin
> will achieve the same results a lengthy tap water rince?
>
> Steve
>
>
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