[Histonet] DAB and beta-galactosidase
|From:||"Serrano del Pozo, Erika" |
Hello Anne, hi histonet list,
I have a problem similar the one you had a couple of years ago in reference
to the DAB and the beta-galactosidase.
I am using in rosa26 mice tissue (that express beta-galactosidase) the
policlonal antibody of Cortex Biochem, and the negative controls, as it
happened to you gives me a positive stain when revealing with DAB... ,
without primary antibody in a possitive tissue and with antibody in a
Were you able to solve your problem?
And does somebody know if this antibody makes positive stain in nucleus or
in cytoplasm (the DAB stain I was talking about gives me possitive in
nucleous, but I have seen articles where common X-gal staining is positive
Can anybody help me, please?!?!?
Thanks in advance.
Centre de Regulaciˇ Gen˛mica
Differenciation and Cancer Programme
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