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Hi Sharon,

Just to clear up any confusion for others, COX can also stand for
CycloOXegenase instead of cytochrome oxidase. I've seen cytochrome
oxidase referred to as CO and CcO. Sorry I cannot address your specific
issues.

Todd


Todd Sherman
President
HistoSoft Corporation
"Biology in a new form..."
Home: www.histosoft.com
Member Services: www.myhistosoft.com

histonet-request@lists.utsouthwestern.edu wrote:
| Today's Topics:
|
|   17. cox and sdh staining (Sharon Cooperman)
| ----------------------------------------------------------------------
Message: 17
Date: Thu, 13 May 2004 18:08:01 -0400
From: Sharon Cooperman 
Subject: [Histonet] cox and sdh staining
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

Dear Histonetters,

I would like to stain for cytochrome oxidase and succinate
dehydrogenase in the same or serial sections from the same animal per
the protocols in John Kiernan's book.  The COX protocol says to
briefly perfuse to get rid of blood and fix in formaldehyde for 15
secs  and then sucrose. The SDH protocol says to use fresh frozen
tissue.  Can I use fresh frozen tissue for the COX protocol and
quickly immersion fix in formaldehyde?  Also, could people direct me
to a good source of high quality formaldehyde or can I use
paraformaldehyde to fix and at what concentration?  I currently use
paraformaldehyde from EMS.  Thanks in advance for answering my
(probably very ignorant, I'm not a histologist) questions.

Sharon
- -- Sharon Cooperman  NIH, NICHD, CBMB 301.435-7735
Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892
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