From: | William Lee |
Histonetters,
I am trying to do the whole mount
in situ hybridization in mouse embryos which are in different stages (7.5-10.5 dpc).
I found that there is high
background after staining with BCIP/NBT. Or, there is even no expression in the
embryos.
I want to ask for optimum protocol for
the Whole mount in situ hybridization of mouse embryo…. Thanks a lot!!
William