|From:||"Montague, Donna C" |
Donna Montague, M.S.
Physiology/Biophysics and Orthopaedic Surgery
University of Arkansas for Medical Sciences
From: William Lee [mailto:email@example.com]
Sent: Thursday, May 23, 2002 9:19 AM
Subject: Asking for optimum protocol of Whole mount in situ hybridization of mouse embryo
I am trying to do the whole mount in situ hybridization in mouse embryos which are in different stages (7.5-10.5 dpc).
I found that there is high background after staining with BCIP/NBT. Or, there is even no expression in the embryos.
I want to ask for optimum protocol for the Whole mount in situ hybridization of mouse embryo.... Thanks a lot!!