From: | "Montague, Donna C" |
Donna Montague, M.S.
Research Associate
Physiology/Biophysics and Orthopaedic Surgery
University of Arkansas for Medical Sciences
(501) 603-1239
-----Original Message-----
From: William Lee [mailto:wlcmwlcm@hkbn.net]
Sent: Thursday, May 23, 2002 9:19 AM
To: histonet@pathology.swmed.edu
Subject: Asking for optimum protocol of Whole mount in situ hybridization of mouse embryoHistonetters,
I am trying to do the whole mount in situ hybridization in mouse embryos which are in different stages (7.5-10.5 dpc).
I found that there is high background after staining with BCIP/NBT. Or, there is even no expression in the embryos.
I want to ask for optimum protocol for the Whole mount in situ hybridization of mouse embryo.... Thanks a lot!!
William