Histonetters,

I am trying to do the whole mount in situ hybridization in mouse embryos which are in different stages (7.5-10.5 dpc).

I found that there is high background after staining with BCIP/NBT. Or, there is even no expression in the embryos.

I want to ask for optimum protocol for the Whole mount in situ hybridization of mouse embryo…. Thanks a lot!!

William