Andrea,

Try a histochemical stain to demonstrate the melanin (a microwave
methenamine silver, Luna's Melanin stain - more sensitive, or a Schmorl's
stain).

Or use immunohistochemistry, HMB-45 marks many melanocytes, though
I've found S100 to be more sensitive.

Regards,
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> <AndreaH@imclone.com> 17/May/2001 07:27am >>>
Dear Histonet,

Can anyone suggest a good antibody and/or method for the staining of
melanocytes?

Thank you in advance.
Andrea





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