Attn: Patsy Ruegg

From:Gayle Callis <>


For some reason there is a circular reference with an alias in your
address, and I cannot reply to you directly, no one elses email addresses
do this to me, so this is coming through HIstonet, sorry folks!  gremlins
in the computer dancing on the keys, messing up address files or some
little thingee ma bob! 

Sigma Dulbeccos PBS, D 5652 cat #, makes up 50 liters.  Make sure you get
the one without magnesium or calcium.

I make up my own TRIS buffered saline (TBS), you can find a recipe for 10X
TBS on website,, just dilute and run with it. I use it at
ph 7.6, and for AP protocols.
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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