I was under the impression that Elmer's white glue was collagen based!
(embarassed) Abizar
www.innogenex.com

Patsy.Ruegg@UCHSC.edu wrote:

> A good way to keep sections on the slides if you are not doing collagen
> markers (the glue interfers with staining) is to precoat the slides (reg.
> Slides) with a solution of 5% elmers type glue in water, dip the slides in
> this then let them air dry.  Pick up your sections onto the glue coated
> slides and dry them as usual, they stick really well, I use this for rabbit
> bones, but beware that glue is collagen based and will cause problems with
> some IHC markers.
> Patsy Ruegg
>
>                 -----Original Message-----
>                 From:   Deb Boswell Lane [mailto:dbl@aretha.jax.org]
>                 Sent:   Wednesday, May 02, 2001 7:46 AM
>                 To:     Histonet@pathology.swmed.edu
>                 Subject:        BrdU in Unicryl??
>
>                 I'm relaying a question from a grad student:
>                 Limulus (horseshoe crab) was embedded in Unicryl and initial
> attempts at
>                 immunohistochemistry for BrdU haven't worked.  Has anyone
> done BrdU immuno
>                 work for light microscopy in Unicryl or similar media?  And
> does anyone
>                 have any suggestions for keeping 10 micron thick paraffin
> sections of
>                 Limulus (horseshoe crab) tissue on slides through IHC
> procedures?  Please
>                 make suggestions!  This if for a dissertation?
>
>                 Thanks in advance!
>
>                 Deb
>                 Deb Boswell Lane
>                 Professional Biomedical Technologist
>                 Biological Imaging
>                 The Jackson Laboratory
>                 600 Main Street
>                 Bar Harbor, Maine 04609
>                 (207) 288-6193
>                 dbl@jax.org