|From:||Abizar Lakdawalla <firstname.lastname@example.org>|
I was under the impression that Elmer's white glue was collagen based!
> A good way to keep sections on the slides if you are not doing collagen
> markers (the glue interfers with staining) is to precoat the slides (reg.
> Slides) with a solution of 5% elmers type glue in water, dip the slides in
> this then let them air dry. Pick up your sections onto the glue coated
> slides and dry them as usual, they stick really well, I use this for rabbit
> bones, but beware that glue is collagen based and will cause problems with
> some IHC markers.
> Patsy Ruegg
> -----Original Message-----
> From: Deb Boswell Lane [mailto:email@example.com]
> Sent: Wednesday, May 02, 2001 7:46 AM
> To: Histonet@pathology.swmed.edu
> Subject: BrdU in Unicryl??
> I'm relaying a question from a grad student:
> Limulus (horseshoe crab) was embedded in Unicryl and initial
> attempts at
> immunohistochemistry for BrdU haven't worked. Has anyone
> done BrdU immuno
> work for light microscopy in Unicryl or similar media? And
> does anyone
> have any suggestions for keeping 10 micron thick paraffin
> sections of
> Limulus (horseshoe crab) tissue on slides through IHC
> procedures? Please
> make suggestions! This if for a dissertation?
> Thanks in advance!
> Deb Boswell Lane
> Professional Biomedical Technologist
> Biological Imaging
> The Jackson Laboratory
> 600 Main Street
> Bar Harbor, Maine 04609
> (207) 288-6193
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