Hi All,
            I work with a rabbit anti- human VWF antibody (factor VIII) (DAKO) at 3.8ug/mL in 10% NBF fixed(24 hours) paraffin embedded mouse tissues Prior to staining, the paraffin-embedded slides for vWF staining were enzyme digested with 0.5% Pronase  respectively for 15 minutes at 37 =B0 C. This is done using a peroxidase based IHC system.
 That being said it would be Very Very Very helpful if people would but specify species they are interested in so that this information is not a was of time....Mouse, Rat, Human, Monkey we are not all in a Clinical Research....
Thanks



Jamie Erickson
Scientist II
Genetics Institute
1 Burtt Rd.
Andover, MA   01810
work : (978) 247-1348
FAX  : (978) 247-1389

>>> Aneeta Patel <apatel@usuhs.mil> 05/02 12:21 PM >>>
hi  all
I am trying to find out if anyone has done factor 8 immunostaining on parafiin embedded tissues and if so can you kindly share the details of method including ag retrival,thanks