Re: factor 8
|From:||Jamie Erickson <JErickson@genetics.com>|
I work with a rabbit anti- human VWF antibody (factor VIII) (DAKO) at 3.8ug/mL in 10% NBF fixed(24 hours) paraffin embedded mouse tissues Prior to staining, the paraffin-embedded slides for vWF staining were enzyme digested with 0.5% Pronase respectively for 15 minutes at 37 =B0 C. This is done using a peroxidase based IHC system.
That being said it would be Very Very Very helpful if people would but specify species they are interested in so that this information is not a was of time....Mouse, Rat, Human, Monkey we are not all in a Clinical Research....
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>>> Aneeta Patel <email@example.com> 05/02 12:21 PM >>>
I am trying to find out if anyone has done factor 8 immunostaining on parafiin embedded tissues and if so can you kindly share the details of method including ag retrival,thanks
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