RE: fixation
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From: | "Weems, Joyce" <JWEEMS@sjha.org> (by way of Histonet) |
To: | HistoNet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
One thing we have found helpful (that I learned from the net years ago) is
to put a xylene between the first two 100% alcohols.
Formalin, graduated alcohols - etc
100%
Xylene
100%
Xylene
Xylene
Paraffin.........
The xylene seems to defat the tissue a little bit
> -----Original Message-----
> From: Mike Bennett [SMTP:bennett1904@piasanet.com]
> Sent: Wednesday, May 17, 2000 6:55 PM
> To: HistoNet@pathology.swmed.edu
> Subject: fixation
>
> This may be a question that is found on this site often but since this
> is new to me I will ask anyway. We recently purchased a new processor
> and I am having some problems with fixation. All my lab has ever used is
> 10% Buffered Formalin and I would like to try something in addition to
> this. I have alot of breast tissue and uteri that is not getting fixed
> properly. (Mushy middles!!) Any suggestions would be appreciated. We
> process about a 12 hour run with the tissues in each solution for a
> least an hour. Yes I have tried to get the Pathologist to work with me
> and cut thinner pieces, but he seems to think they are just fine.
> Thanks!! I am so glad to be part of this. I think this will really be
> helpful to alot of techs out there!
>
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