biotin blocking and non-immune sera for negative control

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From:Sonia Greco <>
To:"'HistoNet Server'" <>
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Dear Histonetters,

I just bought the DAKO Biotin Blocking System, which suggests to use before 
application of the first step of the staining procedure. Does this mean 
after the secondary antibody, before a strepABC and DAB staining. or do I 
apply it before between the primary and secondary antibodies?

Also: I am doing immunohistochemistry with a polyclonal goat anti human 
antibody. For a negative control, I would have liked to use a goat 
non-immune IgG, but didn't have any, so I have used normal goat sera. Would 
you know the best dilution to use to make it a good negative control?

Thanks in advance

QIMR, Australia

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