biotin blocking and non-immune sera for negative control
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From: | Sonia Greco <soniaG@qimr.edu.au> |
To: | "'HistoNet Server'" <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dear Histonetters,
I just bought the DAKO Biotin Blocking System, which suggests to use before
application of the first step of the staining procedure. Does this mean
after the secondary antibody, before a strepABC and DAB staining. or do I
apply it before between the primary and secondary antibodies?
Also: I am doing immunohistochemistry with a polyclonal goat anti human
antibody. For a negative control, I would have liked to use a goat
non-immune IgG, but didn't have any, so I have used normal goat sera. Would
you know the best dilution to use to make it a good negative control?
Thanks in advance
Sonia
QIMR, Australia
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