RE: B&B Gram problem

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From:"Bartlett, Jeanine" <>
To:"'MacDonald, Jennifer'" <>
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Ohmigosh!  I sure have!  I am using it on my automatic coverslipper!  I'll
try hand-coverslipping the next one with my old brand and see what happens.

Thanks so much!

-----Original Message-----
From: MacDonald, Jennifer []
Sent: Tuesday, May 02, 2000 10:40 AM
To: Histonet; 'Bartlett, Jeanine'
Subject: RE: B&B Gram problem

Are you using Richard Allen mounting media?  In the info it states that the
media is not compatible with picric acid stains?  Have you changed your
mounting media?

Jennifer MacDonald

> ----------
> From: 	Bartlett, Jeanine[]
> Sent: 	Tuesday, May 02, 2000 5:59 AM
> To: 	Histonet
> Subject: 	B&B Gram problem
> Histonetters:
> I have developed a problem that I am hoping someone out there in
> "Histoland"
> can help me with.  I have been using Newcomer and Polyscientific 0.1%
> aqueous picric acid/acetone for my Gram procedure for years.  In recent
> weeks a problem has developed.  After completing the picric acid/acetone
> step I have a nice yellowish background.  However, within minutes to hours
> if you go back and look at the coverslipped slide it has turned very pink
> and the there is a yellowish halo or blob that has shifted down the slide
> away from the tissue.  When you look under the scope there is no yellow in
> the tissue section any longer.  I tried a different bottle of picric
> acid/acetone and additional xylene at the end but so far it has not
> helped.
> Has anyone else experienced this problem?
> Thanks in advance for your help.
> Jeanine Bartlett
> Centers for Disease Control
> Atlanta, GA

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