Re: Waterbath adhesive

From:Amos Brooks

    No HALT here :-) We use charged slides and a plain old distilled water
bath with no additives. To prevent wash offs we dry our slides overnight at
50 deg, I have found adhesives to add to background staining. When used with
charged slides it completely defeats the point of using the charged slides.
Also I have been told that the proteinacious material in adhesives (like
HALT) can interfere with an antibody's affinity and avidity, thereby causing
reduced or otherwise unpredictable results. That part I have not directly
observed but it seems logical. My advise: dry the heck out of your slides
before staining them and I swear by charged slides.
Amos Brooks

----- Original Message -----
Sent: Thursday, March 21, 2002 2:58 AM
Subject: Waterbath adhesive

> Hi,
> I'd like to know how many use Halt as an adhesive in their waterbaths.
> you had good luck with it keeping tissues on your slides for IHC.  We have
> ongoing problem with tissues washing, especially needle biopsies.  Any
> information you can give will be appreciated.
> Thanks in advance,
> Ellen Yee
> Sacramento

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