Re: resin embedding for LM

From:Geoff McAuliffe

    Try a sharper knife and a smaller block face. If you are using glass
knives remember that they should be made "fresh", glass is a liquid and flows
over time. I doubt that steel knives will cut epoxy well. Also changing the
angle of the knife and/or the cutting speed might help.

Anila Syed wrote:

> Dear all,
> I have embedded tiny yet valuable, blocks of corpus callosum in TAAB epoxy
> resin, but it seems to be too hard.
> The blocks are juddering and the sections are coming off with horizontal
> lines accross them. Everything is tight enough and held in place.
> Is there a solution to this problem?
> Can I soften the resin in any way?
> Many Thank as always for your help,
> Anila Syed

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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