Fwd: resin embedding for LM

From:"Dr. Ian Montgomery"

        From your description is sounds as if something is loose. Check everything is tight - block in holder, holder in orientation head, head on microtome, knife in carriage. Also make sure the knife edge is properly wetted. Are you using glass or diamond? If glass make sure the edge is good, a blunt knife can produce chatter. How is the instrument with other material and knives from the same batch of glass.         
        Softening the block, a non starter.
Oh, final thought, check the clearance angle.

Date: Thu, 21 Mar 2002 12:55:39 +0000
From: Anila Syed <syedab@totalise.co.uk>
Subject: resin embedding for LM
To: histonet@pathology.swmed.edu

Dear all,

I have embedded tiny yet valuable, blocks of corpus callosum in TAAB epoxy
resin, but it seems to be too hard.
The blocks are juddering and the sections are coming off with horizontal
lines accross them. Everything is tight enough and held in place.
Is there a solution to this problem?
Can I soften the resin in any way?
Many Thank as always for your help,

Anila Syed

Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 332 8855
Office: 4652
Lab: 6644.
e-mail: ian.montgomery@bio.gla.ac.uk

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