Fwd: resin embedding for LM
|From:||"Dr. Ian Montgomery" |
description is sounds as if something is loose. Check everything is tight
- block in holder, holder in orientation head, head on microtome, knife
in carriage. Also make sure the knife edge is properly wetted. Are you
using glass or diamond? If glass make sure the edge is good, a blunt
knife can produce chatter. How is the instrument with other material and
knives from the same batch of
the block, a non starter.
Oh, final thought, check the clearance angle.
Date: Thu, 21 Mar 2002 12:55:39
Dr. Ian Montgomery,
From: Anila Syed <email@example.com>
Subject: resin embedding for LM
I have embedded tiny yet valuable, blocks of corpus callosum in TAAB
resin, but it seems to be too hard.
The blocks are juddering and the sections are coming off with
lines accross them. Everything is tight enough and held in place.
Is there a solution to this problem?
Can I soften the resin in any way?
Many Thank as always for your help,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Tel: 0141 332 8855
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