Microprobe Staining for IHC
From: | "Bennett, Catherine (Katie)" <cbennett@lrri.org> |
I use the Fisher Microprobe station for my IHC staining which uses capillary
action to draw up the solutions onto the slide. Last week, I finished up a
round of slides using the Vector Red cromagen (Alk-phos staining procedure)
with various primary antibodies of interest (vimentin, cytokeratin, c-myc,
MDM2). Many of the tissues have a noticeably increased staining region on
the lower front of the tissue. Kind of like the Vector Red settled due to
gravity and left a gradient of stain, even though the tissues were
completely covered with solution.
I even tried to stain once keeping the slides horizontal during the
cromagen step, but that didn't seem to make that much of a difference.
Anyone seen anything like this before? I never saw this with DAB.
*********************************
Catherine "Katie" Bresee Bennett
Sr. Research Technologist
Lovelace Respiratory Research Institute
Albuquerque, New Mexico
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