Don't know exactly what you are asking but we have on several occasions done
IHC on slides previously stained with H&E.  No need to decolorize before
IHC.  I do remove the counterstain step from the IHC protocol.

Tom Mc Nemar
Pathology Supervisor
Licking Memorial Hospital
Newark, Ohio

> -----Original Message-----
> From:	james.zimmerman@pharma.Novartis.com
> [SMTP:james.zimmerman@pharma.Novartis.com]
> Sent:	Thursday, March 15, 2001 2:06 PM
> To:	Histonet@Pathology.swmed.edu
> Subject:	Double Staining
> 
>      Does anyone have any experience with an H&E plus  an
> immunohistochemical stain with a DAB chromagen .
> 
>                                               Jim Zimmerman
> 
> 
> 
> 
> 
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