Tina,
The following is the protocol supplied with the Vector Methyl Green 
Nuclear Counterstain:
1) Rinse slides in tap water.
2) Stain as follows:
- For individual slides, apply room temp. Vector M.G. solution to 
sections. Incubate slides at 60 degrees C on a slide warmerfor 1-5 
mins. After incubation, rinse slides with ddH2O until rinse water is 
clear.
- For batch processing, preheat Vector M.G. solution to 60 degrees 
in a coplin jar. Add slides and incubate at 60 for 1-5 mins. Rinse as 
above.
3) Wash slides for 1 min in ddH2O. Remove slides and tap to 
eliminate excess water.
4) Dip slides 5-10 times in acetone containing 0.05% (v/v) acetic 
acid.
5)Immediately dehydrate, clear, and permanently mount.
Hope this helps. Greg
Date sent:      	Mon, 19 Mar 2001 15:04:37 +1100
From:           	Tina Cardamone <kcard@myriad.its.unimelb.edu.au>
Subject:        	methyl green as counterstain
Forwarded to:   	DOBBIN@acad1.cs.upei.ca
To:             	HistoNet@pathology.swmed.edu

> Hello,
> 
> I have a simple question regarding methyl green.
> Does anyone have a methyl green counterstain  protocol for 
> immunohistochemistry.
> It sounds simple however I can't seem to find a protocol anywhere.
> 
> Thanks in advance.
> 
> Tina Cardamone
> University of Melbourne.
>