Re: methyl green as counterstain

From:Greg Dobbin <dobbin@Upei.CA>

The following is the protocol supplied with the Vector Methyl Green 
Nuclear Counterstain:
1) Rinse slides in tap water.
2) Stain as follows:
- For individual slides, apply room temp. Vector M.G. solution to 
sections. Incubate slides at 60 degrees C on a slide warmerfor 1-5 
mins. After incubation, rinse slides with ddH2O until rinse water is 
- For batch processing, preheat Vector M.G. solution to 60 degrees 
in a coplin jar. Add slides and incubate at 60 for 1-5 mins. Rinse as 
3) Wash slides for 1 min in ddH2O. Remove slides and tap to 
eliminate excess water.
4) Dip slides 5-10 times in acetone containing 0.05% (v/v) acetic 
5)Immediately dehydrate, clear, and permanently mount.
Hope this helps. Greg
Date sent:      	Mon, 19 Mar 2001 15:04:37 +1100
From:           	Tina Cardamone <>
Subject:        	methyl green as counterstain
Forwarded to:

> Hello,
> I have a simple question regarding methyl green.
> Does anyone have a methyl green counterstain  protocol for 
> immunohistochemistry.
> It sounds simple however I can't seem to find a protocol anywhere.
> Thanks in advance.
> Tina Cardamone
> University of Melbourne.

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