Re: RE: Automation of special stains

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From:Jamie Erickson <>,,,
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I use the Ventana for research with great success I have switch from Ventana reagents to Signet Lab reagents which are much less expensive. I use different  primary and secondary antibody vendors and all is working great. I have not tried special stains but  it would be easy to modify  protocols and times for that.

Jamie Erickson

Jamie Erickson
Associate Scientist
Genetics Institute
1 Burtt Rd.
Andover, MA   01810
work : (978) 247-1348
FAX  : (978) 247-1333

>>> "Fessler, Jim" <> 03/15 2:16 PM >>>
We, here at Pennsylvania Hospital, have the Ventana Special Stains Module.
Although the machine is very easy to use I must admit that the results  seem
inconsistent at times.The reagents are EXTREMELY expensive and expire before
the kit is used completely.  Also, the pre-set  protocols are not  able to
be altered.   
Jim Fessler
Pennsylvania Hospital
Department of Molecular Pathology 
> -----Original Message-----
> From:	Susan Meloan [] 
> Sent:	Wednesday, March 15, 2000 10:24 AM
> To:; 
> Subject:	Re: Automation of special stains
> Last summer we had a demo machine- Ventana Nexus.  I was very skepital
> when I agreed to demo it, but was very surprised at the consistent results
> and ease of use.  I hated to lose the machine when it came time to give it
> up.   The hospital did not budget for the machine, but all in all it was a
> very good asset  while in the lab, and would certainly have automated many
> routine stains, allowing more work per technician.
> Susan Meloan
> Chief Histotechnologist
> Department of Pathology
> Medical College of Georgia
> Augusta, Ga 30912
> (706) 721-3630
> e-mail: 
> >>> "Tom T. McNemar" <> 03/15 7:36 AM >>>
> Hello everyone!  Does anybody have any experience with using automation to
> perform stains like Trichrome, Alcian-Blue Pas, Grocott's Methenamine
> Silver, etc.?  I would appreciate any input you may have.
> Tom
> Licking Memorial Hospital
> Newark, Ohio

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