Yup, you are absolutely right Gayle. I ahve always flushed with Heparin
Saline, before using the fixative....usually 4% para, and usually stored on
ice.....

I cant remember the amount of heparin...(i think it is something like
1.25mls per litre of 25000U/ml..if that seems right..)

I have very rarely had a problem with perfusions.....( i have in the past
used Zambonis fluid as well)

emma carter

> -----Original Message-----
> From:	Gayle Callis [SMTP:uvsgc@msu.oscs.montana.edu]
> Sent:	Wednesday, March 29, 2000 3:55 PM
> To:	histonet@pathology.swmed.edu
> Subject:	RE: perfusion confusion/clotting
> 
> If I remember correctly, heparin (in perfusion fluid?) is used to prevent
> clotting problems.  Linda Jenkins has done this, plus some others, any
> comments
> 
> 
> 
>